Tags: Chemical Compound, Chemical Substance.
Phenyl isothiocyanate (PITC) is a reagent used in reversed phase HPLC. PITC is less sensitive than o-phthaldehyde (OPA) and cannot be fully automated. PITC can be used for analysing secondary amines unlike OPA.It is also known as Edman’s reagent and is used in Edman degradation.Commercially available this compound may be synthesized by reacting aniline with carbon disulfide and concentrated ammonia to give the ammonium dithiocarbamate salt.
