Tags: Enzyme.
In enzymology a polynucleotide 5′-hydroxyl-kinase (EC 2.7.1.78) is an enzyme that catalyzes the chemical reactionATP + 5′-dephospho-DNA ADP + 5′-phospho-DNAThus the two substrates of this enzyme are ATP and 5′-dephospho-DNA whereas its two products are ADP and 5′-phospho-DNA. Polynucleotide kinase is a T7 bacteriophage (or T4 bacteriophage) enzyme that catalyzes the transfer of a gamma-phosphate from ATP to the free hydroxyl end of the 5′ DNA or RNA.