Tags: Chemical Compound, Chemical Substance.
Indo-1 is a popular calcium indicator similar to Fura-2. In contrast to Fura-2 Indo-1 has a dual emissions peak. The main emission peak in calcium-free solution is 475 nm while in the presence of calcium the emission is shifted to 400 nm. It is widely used in flow cytometry.The penta potassium salt is commercially available and preferred to the free acid because of its higher solubility in water.