Guido Ascanio Sforza di Santa Fiora (26 November 1518 — 6 October 1564) was an Italian cardinal known also as The cardinal of Santa Fiora.Born in Rome he was the son of Costanza Farnese and therefore grandson of Pope Paul III brother of Cardinal Alessandro Sforza (1565) uncle of Cardinal Francesco Sforza and great-uncle of Cardinal Federico Sforza (1645).