Galactokinase is an enzyme (phosphotransferase) that facilitates the phosphorylation of α-D-galactose to galactose 1-phosphate at the expense of one molecule of ATP. Galactokinase catalyzes the second step of the Leloir pathway a metabolic pathway found in most organisms for the catabolism of β-D-galactose to glucose 1-phosphate. First isolated from mammalian liver galactokinase has been studied extensively in yeast archaea plants and humans.
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