Tags: Enzyme.
Barbiturase (EC 3.5.2.1) is a zinc containing amidohydrolase. Its systemic name is barbiturate amidohydrolase (3-oxo-3-ureidopropanoate-forming). Barbiturase acts as a catalyst in the second step of oxidative pyrimidine degradation promoting the ring-opening hydrolysis of barbituric acid to ureidomalonic acid. Although grouped into the naturally existing amidohydrolases it demonstrates more homology with cyanuric acid amidohydrolase.