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The Annunciation with St. Margaret and St. Ansanus is a painting by the Italian Gothic artists Simone Martini and Lippo Memmi now housed in the Uffizi Gallery in Florence Italy. It is a wooden triptych painted in tempera and gold with a central panel having double size. Considered Martini’s masterwork and one of the most outstanding works of Gothic painting the work was originally painted for a side altar in the Siena Cathedral.

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