Tags: Enzyme.
The final step of beta oxidation is the cleavage of 3-ketoacyl CoA by the thiol group of another molecule of Coenzyme A. This reaction is catalyzed by Acetyl-CoA C-acyltransferase (or Β-ketothiolase). The thiol is inserted between C-2 and C-3 which yields an acetyl CoA molecule and an acyl CoA molecule which is two carbons shorter. Acetyl-CoA C-acyltransferase belongs to the thiolase family of enzymes.