Tags: Enzyme.
In enzymology a 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase (EC 1.14.13.95) is an enzyme that catalyzes the chemical reaction7alpha-hydroxycholest-4-en-3-one + NADPH + H+ + O2 7alpha12alpha-dihydroxycholest-4-en-3-one + NADP+ + H2OThe 4 substrates of this enzyme are 7alpha-hydroxycholest-4-en-3-one (7 alpha-hydroxy-4-cholesten-3-one NADPH H+ and O2 whereas its 3 products are 7alpha12alpha-dihydroxycholest-4-en-3-one NADP+ and H2O.This enzyme belongs to the family of oxidoreductases specifically those acting on paired donors with O2 as oxidant and incorporation or reduction of oxygen.